The charity - which helps 110,000 children in 383 projects across the UK each year - said it would have a 'constant stream' of celebrity and designer items for sale.
Barnardo's head of retail Gerard Cousins said he was 'incredibly excited' about the interest in donating to the shop from BBC's Jonathan Ross, model Liz Hurley and actress Dame Helen Mirren.
Barnardo's store in Marylebone is to open on November 16 and will be 'a hotspot for any discerning shopper', according to Cousins.
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