Customers aged between 30 and 65 will be able to buy a pack of four tablets for£50, after a consultation with a pharmacist and various health checks.
Until now, men wishing to take Viagra have only been able to obtain it by prescription from a doctor.
However, if customers wish to buy regular supplies of the medicine they will need to consult a private doctor, at a cost of£37.50.
The sale of Viagra will begin in three Boots stores in Manchester, before being made available in stores across the UK.
A Boots spokeswoman said: 'There are a number of saftey checks in place to ensure safe and correct supply. Erectile dysfuntion can be a marker for an underlying medical condition, so we are keen to take this extra step.'
Viagra is estimated to be taken by about 27 million men worldwide, including 900,000 in the UK.
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